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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
15/01/2015 |
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Data da última atualização: |
15/01/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MAGALHAES, A. B.; VILLAS-BOAS, P. R.; BRIGANTI, R.; RANULFI, A. C.; CAMPOS, L. J. M.; MEYER, M. C.; HIROSE, E.; NETO, L. M.; MILORI, D. M. B. P. |
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Afiliação: |
PAULINO RIBEIRO VILLAS BOAS, CNPDIA; LADISLAU MARTIN NETO, DE/P&D; DEBORA MARCONDES BASTOS P MILORI, CNPDIA. |
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Título: |
Distinção nutricional entre folhas de soja sadias e com sintoma de soja louca II utilizando a técnica espectroscopia de emissão óptica com plasma induzido por laser. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 293-296. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana. |
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Idioma: |
Português |
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Notas: |
Entrada padronizada: MARTIN-NETO, L. |
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Palavras-Chave: |
Instrumentação - Evento. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/115580/1/293siagro-2014-print01.pdf
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Marc: |
LEADER 00935nam a2200169 a 4500
001 2005631
005 2015-01-15
008 2014 bl uuuu u00u1 u #d
100 1 $aMAGALHAES, A. B.
245 $aDistinção nutricional entre folhas de soja sadias e com sintoma de soja louca II utilizando a técnica espectroscopia de emissão óptica com plasma induzido por laser.
260 $aIn: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 293-296. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.$c2014
500 $aEntrada padronizada: MARTIN-NETO, L.
653 $aInstrumentação - Evento
700 1 $aVILLAS-BOAS, P. R.
700 1 $aBRIGANTI, R.; RANULFI, A. C.; CAMPOS, L. J. M.; MEYER, M. C.; HIROSE, E.
700 1 $aNETO, L. M.
700 1 $aMILORI, D. M. B. P.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MACHADO, G. M.; CAIXETA, E. S.; LUCCI, C. M.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Zygote, v. 20, p. 123-134, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. MenosThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demon... Mostrar Tudo |
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Palavras-Chave: |
Cultura do embrião; Sexagem. |
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Thesagro: |
Gado. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179338/1/posthatching-development-of-bovine-embryos-in-vitro-the-effects-of-tunnel-preparation-and-gender.pdf
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Marc: |
LEADER 02172naa a2200217 a 4500
001 1950734
005 2023-03-07
008 2011 bl uuuu u00u1 u #d
100 1 $aMACHADO, G. M.
245 $aPost-hatching development of bovine embryos in vitro$bthe effects of tunnel preparation and gender.$h[electronic resource]
260 $c2011
520 $aThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression.
650 $aGado
653 $aCultura do embrião
653 $aSexagem
700 1 $aCAIXETA, E. S.
700 1 $aLUCCI, C. M.
700 1 $aRUMPF, R.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tZygote$gv. 20, p. 123-134, 2011.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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